5- HT Positive en erochromaffin cell from the GL tract and examination of how vesicular 5-ht release is affected by age.

5- HT Positive en erochromaffin cell from the GL tract and examination of how vesicular 5-ht release is affected by age.An Optimization of a technique for the isolation of 5- HT Positive en erochromaffin cell from the GL tract and examination of how vesicular 5-ht release is affected by age.Aim of the inv estigation:To optimize EC cells isolation from mouse colonTo characterize the effects of age on 5-HT releaseproperties from murine enterochromaffin cells using electro chemical-sensingMain objectives1) To investigate and understand thefunction of EC cells in GI tract2% The way in which motility occurs with age and the role of EC cell in this.3 The role of serotonin onthese cellsSpecific objectiveEx animating different age group mouse of 3, 18 and 24 Months by;1) Ev oke inherent mucosal reflexesgestehd by brush stimulation or circumferential stretch. (To understand the changes occur in proximal and distal colon when stimulatedrus 2) Recording from electrical field stimulation of segments of intact distal colon with and without mucosa.:

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